Departing on October 4, Prescott, along with 30 other participants will swim the Humber, cycle from Hull to Liverpool, where they will swim the Mersey, before running to Runcorn.
The final leg of the demanding 48 hour event will see the group canoe up the Bridgewater canal to Old Trafford in time for the Stobart Super League Grand Final on Saturday 6 October.
Backed by Engage Mutual, the aim of the challenge is to raise over £30,000 for The Steve Prescott Foundation, set up by the former England international following his diagnosis with cancer in 2006.
The foundation provides funding to The Christie cancer hospital in Manchester, where Steve has undergone extensive treatment. The second charity to benefit is the Try Assist Benevolent Fund, which supports former rugby players whose lives are affected following serious injury.
Speaking of the challenge Steve Prescott said:
“We’ve undertaken a number of challenges over the last few years but this will be the toughest not just in terms of the activities but the timescales involved.
“I’d like to thank everyone involved for committing themselves to such a gruelling event in order to raise money for good causes.”
This is the second challenge which Prescott will have undertaken in 2012 following his Paris to London: Marathon Challenge which he successfully completed with ex-Great Britain Captain Paul Sculthorpe earlier this year.